Software LS-MIDA for efficient mass isotopomer distribution analysis in metabolic modelling
نویسندگان
چکیده
منابع مشابه
Measuring protein synthesis by mass isotopomer distribution analysis (MIDA).
The measurement of protein kinetics by isotopic techniques has been hindered by the long-standing difficulty of accurately measuring the isotope content of the biosynthetic precursor pool (aminoacyl-tRNA in tissues). Mass isotopomer distribution analysis (MIDA) is a stable isotope-mass spectrometric (MS) technique for measuring biosynthetic precursor enrichments from measurements on a polymeric...
متن کاملIsotopomer measurement techniques in metabolic flux analysis II: mass spectrometry.
Mass spectrometry (MS) offers a sensitive, reliable, and highly accurate method for measurement of isotopic labeling, which is required for generating comprehensive flux maps using metabolic flux analysis (MFA). We present protocols for assessing isotope labeling in a wide range of biochemical species, including proteinogenic amino acids, free organic and amino acids, sugar phosphates, lipids, ...
متن کاملOpenFLUX: efficient modelling software for 13C-based metabolic flux analysis
BACKGROUND The quantitative analysis of metabolic fluxes, i.e., in vivo activities of intracellular enzymes and pathways, provides key information on biological systems in systems biology and metabolic engineering. It is based on a comprehensive approach combining (i) tracer cultivation on 13C substrates, (ii) 13C labelling analysis by mass spectrometry and (iii) mathematical modelling for expe...
متن کاملComparison of deuterium incorporation and mass isotopomer distribution analysis for measurement of human cholesterol biosynthesis.
To compare endogenous cholesterol biosynthesis measured by deuterium incorporation (DI) and mass isotopomer distribution analysis (MIDA), cholesterol fractional and absolute synthetic rates were measured simultaneously by both techniques under identical physiological conditions. Twelve subjects (22 to 39 years of age) underwent a dual stable isotope protocol, involving oral deuterium oxide admi...
متن کاملA critical evaluation of mass isotopomer distribution analysis of gluconeogenesis in vivo.
There are conflicting reports concerning the reliability of mass isotopomer distribution analysis (MIDA) for estimating the contribution of gluconeogenesis to total glucose production (f) during [13C]glycerol infusion. 1 We have evaluated substrate-induced effects on rate of appearance (Ra) of glycerol and glucose and f during [2-13C]glycerol infusion in vivo. Five groups of mice were fasted fo...
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ژورنال
عنوان ژورنال: BMC Bioinformatics
سال: 2013
ISSN: 1471-2105
DOI: 10.1186/1471-2105-14-218